PREPARATION OF FERRIC OXIDE NANOPARTICLES AND EVALUATION OF
ANTIOXIDANT PROPERTY IN CEREBRAL ISCHEMIA REPERFUSION MODEL

Shikha S, Neha K, Dimple C

Department of Pharmaceutical Sciences and Research, Punjabi University, 147002, Patiala, India

The present study aims to prepare ferric oxide nanoparticles and to investigate its antioxidant property in reperfusion induced cerebral injury. The mean hydrodynamic diameter of obtained ferric oxide nanoparticles with change in molar ratios of Fe2+/Fe3+ from 0.5 to1 was found to be 174.4nm and 313.3nm respectively.The mean hydrodynamic diameter of PVP coated ferric oxide nanoparticles was found to be 469.3nm. Ferric oxide nanoparticles showed a negative zeta potential value of 10.8mV and PVP coated ferric oxide nanoparticles was -11.3mV. X-Ray diffraction showed average crystalline size of 17nm and 41nm for uncoated and PVP coated ferric oxide nanoparticles. There was a dose dependent increase in the percentage antioxidant activities as measured by modified DPPH method.Ferric oxide nanoparticles (10mg/kg and 20mg/kg, 1 ml i.p.  doses of uncoated and PVP coated) were administered before cerebral ischemia. Global cerebral ischemia was induced by bilateral carotid artery occlusion for 15 minutes followed by reperfusion for 24 hrs. After 24 hrs animals were sacrificed and the antioxidant effect of uncoated and PVP coated ferric oxide nanoparticles was determined.Cerebral infarct size was estimated using triphenyltetrazolium chloride (TTC) staining, thiobarbituric acid reactive substances and glutathione levels. Administration of ferric oxide nanoparticles significantly reduced cerebral infaract size. Thus, ferric oxide nanoparticles have a great potential in reducing incidence of global cerebral  ischemia.