Liposome loaded with Resveratrol and targeted using the natural ligand, transferrin, to Glioblastoma cells
National Institute of Pharmaceutical Education and Research Uttar Pradesh Raebareli, India
Priyanka Gresess Anand
Integral University, Lucknow India
Glioblastomas are highly aggressive and dynamic malignant type of primitive tumor originates inherently inside the brain. Customarily, it is considered as the most distructive human cancer with only few years of survival after its detection. Commonly used chemotherapeutic agents are frequently lead to drug resistance and result in exhibiting varied toxicities in normal tissue due to their off target effects. Resveratrol, a natural source polyphenol suggests numerous benefits to human health has been claimed to be effective in gaining cytotoxic response throughout the cancer stage irrespective of their initiation, promotion and progression. Nevertheless, the exploration of inferior physiochemical character of Resveratrol strictly resists its utility as a free drug, and to enhance its solubility and stability status. In literature study, resveratrol was encapsulated into PEGylated liposomes (RES-L) to nullify its previously found limitations. Since transferring receptors (TfRs) are overexpressed in majority of cancers, the liposomal surface was altered with transferrin moieties (Tf-RES-L) to make them more specific towards cancerous cells. The liposomal nanomedicines developed in research were focusing to aids in intensifying the physico-chemical behaviour of resveratrol and exploiting the diverse form of targeting potential of liposomes to efficiently counteract Glioblastoma cells.The RES-L were reported to be fairly stable, high drug loading capacity, sustained drug-release in vitro. Flow cytometry and confocal microscopy were used to study the association with, and internalization, The Tf-RES-Ls were significantly more cytotoxic as compared to free Resveratrol or RES-L. Resveratrol formulations were evaluated in a subcutaneous xenograft mouse model of Glioblastoma and in tumor growth inhibition investigation and a modified survival study, Tf-RES-L were notified to be more prominent in their ability to hinder tumor growth and improve survival probability in mice.