Shiva K. Rastogi,a,b,* CharLene M. Gibson,a Josh R. Branen,a
D. Eric Aston,c A. Larry Branen,a and Patrick J. Hrdlickab,*
a Biosensors and Nanotechnology Applications Laboratory, University of Idaho, Coeur d’Alene, ID 83814-2277, USA.
b Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA.
c Department of Chemical and Materials Engineering, University of Idaho, Moscow, ID 83844-1021, USA.
A lateral flow test strip assay, enabling sensitive detection of DNA specific to the food-borne pathogen Escherichia coli O157:H7, is described. The use of LNA-conjugated gold nanoparticle probes, along with signal amplification protocols, results in minimum detectable concentrations of ~0.4 nM.