A NOVEL ‘GREEN’ SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLES (SNP) USING LAGENARIA VULGARIS LEAF EXTRACT

Rahul S. Adnaik, Pratibha S. Gavarkar, Shrinivas K. Mohite, Chandrakant S. Magdum

Rajarambapu College of Pharmacy, Kasegaon Tal. Walwa, Dist. Sangli 415404, Maharashtra, India

Rahul S. Adnaik, M. Pharm (Pharmacology): Studied at Shivaji University, Kolhapur. Asst Professor at Rajaramabpu College of Pharmacy, Kasegaon, MS, India. Five years of teaching and Research experience. Published 24 research papers in reputed National and International Journals and 2 books. Research interest includes neuropharmacology and herbal drug formulation.Presently pursuing Ph D.

Nanotechnology has opened new opportunities in the field of medical sciences and pharmaceuticals. Nanoparticles are being viewed as fundamental building blocks of nanotechnology. Metal nanoparticles have tremendous applications in the field of high sensitivity biomolecular detection and diagnostics, antimicrobials and therapeutics, catalysis and micro-electronics. Green synthesis is the safe and easiest method of producing silver nanoparticles. The silver nanoparticles found to have the antibacterial activity because of the production of the silver ions. In the present study the green nanoparticles were prepared by using ethanolic leaf extract of Lagenaria vulgaris.  The synthesis of silver phyto nanoparticles were prepared by adding silver nitrate solution to the plant extract. Nanoparticles were characterised using UV-Visible absorption and FTIR studies.  The silver phyto nanoparticles were tested for antimicrobial activity. The test cultures included in this study were Staphylococcus aureus, E. Coli and Pseudomonas aeruginosa. The antibacterial activities of nanoparticles showed maximum inhibitory rate using 150μg of these plants extract. The silver nanoparticles from herbal leaves showed a good antibacterial activity than the plants used. Further studies needed to find out the efficacy, longevity and toxicity to improve the current investigation.  This is the first attempt of introducing silver and gold phytonanoparticle isolation and antimicrobial screening of  Lagenaria vulgaris.