Metal Nanoparticles Reduce Intracellular HIV-1 Replication and Stimulate Growth of HIV-1  Infected PBMC

Dr- S-K. Prakash

NAVAL AIDS RESEARCH CENTER

Background:

Noble nanoparticles have the potential to be used as therapeutic agents in HIV-1 infection. Noble nanoparticles have higher electron resonance potential than  organic drug molecules to arrest HIV and enhance cell growth. Silver nanoparticles have been shown to exhibit promising cytoprotective activities towards HIV-infected T-cells and arrest  HIV by blocking gp120-CD4 interaction. Metal nanoparticles are engineered as non toxic and employed as it have unique signal propagation properties. Combined nanoparticles may arrest HIV replication by functionalize with variety of molecules and produce specific signals and proliferate PBMC by inducing cytokines. The aim is to examine the effect of combined nanoparticles on growth and viral replication in HIV-1 infected human PBMC.

Methods

Combinations of Silver, Tin and Zinc (AgSnZn) nanoparticles were processed as nanomedicine (NM), 28nm in size(less than 25nm is toxic) to treat HIV-1 infected cells in cell culture. PBMC cells were isolated from HIV-1 positive laboratory sample and cultured with RPMI 1640 medium, IL-2, PHA and 10%FCS in 96 well plates at the cell density of 2.5 × 105 cells/ml with NM and without NM for 96 hrs. The Immuno Peroxidase Test (IPT), an intracellular viral antigen detection assay to assess  intracellular antigen and cell quantification assay (CQA) to quantify  cells were employed in both  the cultures  to find intracellular HIV and  to assess cell growth. Experiments were repeated ten times.

Results

The  cells quantity in NM treated cultures had more population (10x105 cells/ml) than the non treated cells (4.5x105 cells/ml ),which was 55% (p˂0.0001,t-test  of ten assays) higher shows that NM has induced  cell growth. IPT revealed absence of HIV (no golden granules) in cytoplasm of NM treated cells but present in non NM treated cells which indicate the NM has arrested the intracellular HIV (p˂0.001,Chi-Squired test of ten assays).This results show that the NM can arrest HIV and improve cell count.

Conclusion

This study has revealed that the NM arrests the intracellular HIV and proliferate PBMC as well. This NM could be considered for therapeutic approach to HIV-1 infected individuals. Further studies are to be carried out to characterize the molecular mechanisms of the effects.

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