The objective of the present investigation was to enhance the bioavailability of practically insoluble Curcumin by preparing aqueous bioadhesive nanosuspension. The nanosuspension was prepared by suspending in water soluble polymer hydroxypropyl methylcellulose (HPMC) and docusate sodium matrix followed by nanosizing using media or wet milling technique using heavy zirconium oxide beads and further planned to functionalize the Curcumin nanoparticles with Hyaluronic acid polymer to formulate into target drug delivery system for rheumatoid arthritis (RA). The resultant nanosuspension of Curcumin was evaluated for particle size, sedimentation volume, pH, viscosity, saturation solubility and physical stability by centrifugation. Curcumin nanosuspension showed particle size of 95.5±5.4 nm and 4.95 cP while pH 4.01, showing more than 40 times increases in solubility. Furthermore, physical stability of the Curcumin nanosuspension was evaluated by centrifugation and found to be stable. Thus, the results conclusively demonstrated a media milling technique is suitable to manufacture high bioavailable and physically stable Curcumin nanosuspension for target drug delivery system.