EFFECTIVE ENTRAPMENT OF DRUG SERRATIOPEPTIDASE
IN TRANSFEROSOMES AND ASSESSING ITS TRANSDERMAL
DELIVERY

Santhosh K, Shankar T, Balchandar N

Department of Physics & Nanotechnology, SRM University, Kattankulathur.

 
Drug delivery is a process of administering a pharmaceutical compound to achieve therapeutic effect in humans or animals for effective disease  treatment. Transdermal drug delivery is one of the efficient ways of drug administration with major significance on patience compliance and to reach deep inside the systemic circulation in short time interval.
Transfersomes are artificial nano vesicles designed like a cell vesicle suitable for control and targeted drug delivery for transdermal mode. These transferosomes has significant property of size and shape flexibility which especially consider for assessing its transdermal mode of administration. Study was aimed to administer the anti-inflammatory drug called Serratio peptidase which is used in the treatment of swelling and bronchitis was delivered transdermally by a new protocol. Amount of serratio peptidase was quantified by Standard protein estimation method (Lowry’s method) and transfersome synthesis was done. Estimated amount of protein was encapsulated into the vesicle. Perhaps entrapment efficiency of conventional transdermal drug delivery vesicles, initially found to be 20% and it was optimized to increase the entrapment efficiency. We were able to achieve a higher efficiency of about 78%. The drug releasing kinetics was tested on goat’s skin and characterization was done using UV Spectrophotometer and SEM. Hence, the methods and techniques adopted to increase the entrapping efficiency are discussed in detail.