Bioactive Silver Nanoparticles from Endophytic Fungus Fusarium semitectum and its Antibacterial activity

Ashish Kumar Singh, Dattu Singh and Vandana Rathod

Gulbarga University Kalaburagi

Nanotechnology has become a platform to alter and modify and develop the important properties of metal in the form of nanopaticles with its promising applications in various fields for the benefit of mankind. In the present study endophytic fungus Fusarium semitectum. was isolated from healthy leaves of Withania somnifera (Ashwagandha) and was subjected for the extracellular biosynthesis of silver nanoparticles (AgNps). The synthesized AgNps were characterized using Visual observation, UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). Further AgNps synthesized were tested for their efficacy against the bacterial pathogens like E.coli, S.typhi and S.aureus. The formation of AgNps was confirmed by visual observation by change in colour from pale white to brown and the Surface Plasmon Resonance was determined by UV-Vis spectra at 422 nm. TEM revealed the formation of small sized spherical shaped nanoparticles ranging 12-20 nm. Antibacterial activity of AgNps against E.coli, S.typhi and S.aureus showed encouraging results, showing maximum zone of inhibition of 26mm, 26mm and 28 mm respectively at 60μl concentration of AgNps against E.coli, S.typhi and S.aureus.

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