D. Inbakandan1,4, V. Sivakumar2, Dune Naga Ravindra2, C. Kumar1, R. Venkatesan3, S. Ajmal Khan4

  1. Centre for Ocean Research, Sathyabama University, Chennai 600 119, India
  2. Department of Biotechnology, Sathyabama University, Chennai 600 119, India
  3. National Institute of Ocean Technology, Pallikaranai, Chennai-600036, India
  4. Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608 502, India

A simple wet-chemical technique for the assembly of silver nanopartilces on conventional metal substrates, which alleviates the need for lithography, has been developed. Silver nanoparticles were prepared by the biological reduction of silver precursors and attached to silane functionalized Stainless steel surfaces to create a Self Assemble Monolayer with anti-microfouling properties. The assemblies of silver nanoparticles on surfaces were characterized by Scanning electron microscopy and Energy Dispersive X-ray Spectroscopy. The silver nanoparticles diameter was estimated by TEM analysis. The nanoscale nature of the surface undulations, have been observed and compared by scanning electron microscopic images of surfaces. Detection and measurement of the energy permits elemental analysis of surface revealed the coating nature and silver nanoparticles deposition. Contact angle measurements revealed the hydrophobic nature and surface energy of the prepared surfaces. This work reported the marine biofilm formation on surfaces exposed to seawater in vitro for 10 days. After retrieval from seawater the surfaces were scanned with electron microscope and the images are compared for marine biofilm formation. Biofilm bacterial attachments on the surfaces were studied by acridine orange staining and live/dead Baclight fluorescence staining. Low marine biofilm formation and hydrophobic nature qualifies the modified surface for marine antifouling applications.



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