Green Synthesis of Silver Nanoparticles Using Ocimum Sanctum (Tulsi) Leaf Extract and Evaluation of their Antimicrobial Activities

Jagpreet Singh, Deepak Kukkar, Mohit Rawat

Sri Guru Granth Sahib World University (Punjab)

There is an increasing demand of silver nanoparticles due to their unique properties and applications in various fields such as medicine, catalysis, nanoelectronics, textile field, pollution and water treatment. Nanosilver strong antimicrobial activity is the major reason for the development of nanosilver containing products. The silver nanoparticles are synthesis by various chemical and physical methods. The major drawbacks of chemical and physical methods are the synthesis processes are expensive and many toxic chemicals are used. To overcome these problems Green Nanotechnology comes to play very crucial role for synthesis of silver nanoparticles. Use of various plant for synthesis of biogenic silver nanoparticles referred as Green Nanotechnology. In the present study ,we reported the green synthesis of silver nanoparticles using by Ocimum sanctum (Tulsi) leaf extract in the exposure direct sunlight atmosphere without using any chemical additive . The Ocimum sanctum (Tulsi) leaf extract act as reducing agent as well as capping agent.Developed Ag nanoparticles were duly characterized and tested for their antibacterial activity. The formation of silver nanoparticles was observed by the change of colour from colourless to brown by the addition of Ocimum sanctum (Tulsi). UV-Vis absorption spectroscopy was used to monitor the quantitative formation of silver nanoparticles . FTIR analysis revealed that the AgNPs were stabilized by eugenols, terpenes, and other aromatic compounds present in the extractAntimicrobial activity of the silver bio-nanoparticles was performed by a well diffusion method. The morophology of the Silver Nanoparticles study by Scanning Electron Microscopy

Keywords: Green Synthesis, Tulsi Leaves, Antimicrobial Activity, AgNps