Title: Escheriosome-mediated cytosolic delivery of PLK1-specific siRNA: potential in treatment of liver cancer in BALB/c mice

Authors: Arun Chauhan, Swaleha Zubair, Ahmad Nadeem, Sajid Ali Ansari, Mohammad Yunus Ansari & Owais Mohammad

Abstract:

Aim: In the present study, the anticancer efficacy of a novel escheriosome-based formulation of polo-like kinase enzyme (PLK1)-specific siRNA was evaluated against liver cancer in BALB/c mice. Materials & methods: The escheriosome-based siRNA nanoparticles were prepared using lipids isolated from Escherichia coli. The escheriosomes were characterized for size, surface charge and stability. The anticancer potential of PLK1-specific siRNA formulation was ascertained on the basis of expression of pro-/anti-apoptotic factors and histopathological studies. Results: The escheriosome-entrapped siRNA was found to be released in surrounding milieu in a sustained manner. The nanoformulation was successful in modulating proapoptotic factors and eventually helped in better survival of the treated animals. Conclusion: Our data demonstrate the efficacy of systemically administered siRNA in the treatment of experimental liver cancer. This novel therapeutic strategy may be applicable to a broad range of cancers in patients with the obstinate form of the disease.

Direct link: http://www.futuremedicine.com/doi/abs/10.2217/nnm.13.21