The 3rd edition of the International Winter School on Nano and Biotechnology, co-organized by the Latemar consortium (www.latemar.polito.it), the Education, University and Research Department of the Trento Province (PAT), and the Trentino System of Higher Education and Research (STAR: University of Trento, Foundations and Research Centers) is focused on Next-Generation DNA sequencing technologies. “-omics” approaches and biomedical research benefit from recent advances and novel techniques for high-throughput analysis.