Cells and Stuff Volume 1.pdfOne of our partner schools UWRF is involved in tissue engineering. The PDF document describes some of the activities the students are involved with including stem cells, 3D tissue culturing and cancer research. Dr. Timothy Lyden has held workshops in his Tissue and Cellular Innovation Center (TCIC) for our students during the past two years. Students inoculate scaffolding material with stem cells and culture them for a period of time. Using a three dimensional scaffolding material allows the cells to differentiate into complex structures. These tissues are then prepared for SEM analysis using standard biological sample preparation methods of fixing with gluteraldehyde, dehydrating, critical point drying, sputter coating and finally imaging in the SEM.