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Cell growth in 3D composite-polymer scaffolds. 3D scaffolds by sequential DLW consisting of PEG-DA/PETA (4.8%) frameworks and Ormocomp cubes. Primary chicken fi broblasts are cultivated in the scaffolds and immunostained for fi bronectin (red), f-actin (green), and paxillin (yellow). a) Top view of a confocal image stack depicting cells adhering to one, two, or three fi bronectin-positive Ormocomp cubes. b) Top view and c) 3D reconstruction of a confocal image stack depicting a single cell adhering to Ormocomp cubes in different heights. d–i) Single confocal sections of a cell adhering to seven Ormocomp cubes. d) PEG-DA autofl uorescence, e) differential interference contrast (DIC), f) fi bronectin immunofl uorescence, g) f-actin fi bers, h) paxillin immunofl uorescence, and i) overlay of all channels. j–l) 3D reconstruction of a confocal image stack of the cell shown in (d–i). Different oblique views with or without the PEG-DA scaffold demonstrate 3D cell spreading under geometrical control. The arrow is pointing in the same direction in all images. Scale bar in (f) also applies to (d–i). Scale bar in (l) applies to (j–l).

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Aashis.S.Roy July 3, 2013 at 8:45am

Cell growth in 3D composite-polymer scaffolds. 3D scaffolds by sequential DLW consisting of PEG-DA/PETA (4.8%) frameworks and Ormocomp cubes. Primary chicken fi broblasts are cultivated in the scaffolds and immunostained for fi bronectin (red), f-actin (green), and paxillin (yellow). a) Top view of a confocal image stack depicting cells adhering to one, two, or three fi bronectin-positive Ormocomp cubes. b) Top view and c) 3D reconstruction of a confocal image stack depicting a single cell adhering to Ormocomp cubes in different heights. d–i) Single confocal sections of a cell adhering to seven Ormocomp cubes. d) PEG-DA autofl uorescence, e) differential interference contrast (DIC), f) fi bronectin immunofl uorescence, g) f-actin fi bers, h) paxillin immunofl uorescence, and i) overlay of all channels. j–l) 3D reconstruction of a confocal image stack of the cell shown in (d–i). Different oblique views with or without the PEG-DA scaffold demonstrate 3D cell spreading under geometrical control. The arrow is pointing in the same direction in all images. Scale bar in (f) also applies to (d–i). Scale bar in (l) applies to (j–l).

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