My research field or area of interest innanotechnology
Director,Institute for Applied Envieronmental Biotechnology Innovation,Bhubaneswar,751016,India
1. Biological sequestration and retention of cadmium as CdS nanoparticles by the microalga Scenedesmus-24 Jayashree Jena, Nilotpala Pradhan , V. Aishvarya, Rati Ranjan Nayak, Bisnu Prasad Dash,Lala Behari Sukla, Prasanna Kumar Panda, Barada Kanta Mishra, JOURNAL OF APPLIED PHYCOLOGY • DECEMBER 2014 with 65 READS Impact Factor: 2.56 • DOI: 10.1007/s10811-014-0499-8, pp 1-10 ABSTRACT: Biological sequestration of cadmium (Cd) and retention of adsorbed cadmium as cadmium sulphide (CdS) nanoparticles inside the cell by a lipid-producing green algae Scenedesmus-24 is reported. The microalga was able to grow in the growth media containing 30 mg L−1 of cadmium without any growth inhibition. Adsorption of Cd(II) was dependent on the pH of the medium, initial concentration of cadmium, density of algal biomass (biosorbent dose), and contact time. The adsorption follows Langmuir isotherm pattern with an estimated maximum cadmium adsorption capacity at 50 mg g−1. The kinetics of adsorption followed Lagergren’s pseudo-second-order model. FTIR analysis revealed the presence of different functional groups on the algal biomass which may be responsible for adsorption of Cd(II). After adsorption, the bound metal ions were retained in the microalgal biomass as CdS nanoparticles. Presence of CdS nanoparticle was confirmed by XRD and TEM analysis. The results of the present study conclusively demonstrate that the microalga Scenedesmus-24 may be a promising candidate for sequestration of cadmium from cadmium polluted water and also its recovery as precious CdS nanoparticles. 2. Microalga Scenedesmus sp.: A Potential Low-Cost Green Machine for Silver Nanoparticle Synthesis Jayashree Jena • Nilotpala Pradhan • Rati Ranjan Nayak • Bishnu Prasad Das • Lala Behari Sukla • Prasanna Kumar Panda •Barada Kanta Mishra, Journal of Microbiology and Biotechnology 01/2014; 24(4). DOI:10.4014/jmb.1306.06014 • 1.53 Impact Factor, PP- 522-33 ABSTRACT: Bionanotechnology has revolutionized the nano material synthesis by providing a green synthetic platform using its biological systems. Among the biological systems microalgae has tremendous potential to uptake metal ions and produce nanoparticle by detoxification process. The present study explores the intracellular and extracellular biogenic synthesis of silver nanoparticles (SNPs) using a unicellular green microalga Scenedesmus. Biosynthesized SNPs were characterized by AAS, UV-Vis spectroscopy, TEM, XRD, FTIR, DLS, TGA studies and finally checked for antibacterial activity. Intracellular nanoparticle biosynthesis is initiated by high rate of Ag(+) ion accumulation in the microalgal biomass and subsequent formation of spherical crystalline SNPS (of average size of 15-20 nm) due to biochemical reduction of Ag(+) ions. The synthesized nanoparticles were intracellular as confirmed by the UV-Vis spectra of experimental medium. Furthermore, extracellular synthesis using boiled extract shows formation of well scattered, highly stable, spherical SNPs with average size of 5-10 nm. Size and morphology of the nanoparticle was confirmed by TEM. Crystalline nature of the SNPs was evident from the diffraction peaks of XRD and bright circular ring pattern of SAED. FTIR and UV-Vis spectra showed that the biomolecules, proteins and peptides, are mainly responsible for formation and stabilization of SNPs. Further, the synthesized nanoparticles exhibited high antimicrobial activity against pathogenic gram negative and gram positive bacteria. Use of such a microalgal system provides a simple, cost effective alternative template for biosynthesis of nanomaterials in large scale system with a great use in biomedical application. 3. Controlled Synthesis of Gold Nanoparticles Using Aspergillus terreus IF0 and Its Antibacterial Potential against Gram Negative Pathogenic Bacteria, Eepsita Priyadarshini • Nilotpala Pradhan • Lala Behari Sukla •Prasanna K. Panda • B. K. Mishra Journal of Nanotechnology 01/2014; Volume 2014, Article ID 653198:PP-1-9. DOI:10.1155/2014/65319 ABSTRACT: Biosynthesis of monodispersed nanoparticles, along with determination of potential responsible biomolecules, is the major bottleneck in the area of bionanotechnology research.he present study focuses on an ecofriendly, ambient temperature protocol for size controlled synthesis of gold nanoparticles, using the fungus Aspergillus terreus IF0. Gold nanoparticles were formed immediately, with the addition of chloroauric acid to the aqueous fungal extract. Synthesized nanoparticles were characterized by UV-Vis spectroscopy, TEM-EDX, and XRD analysis. Particle diameter and dispersity of nanoparticles were controlled by varying the pH of the fungal extract. At pH 10, the average size of the synthesized particles was in the range of 10–19 nm. Dialysis to obtain high and lowmolecular weight fraction followed by FTIR analysis revealed that biomolecules larger than 12 kDa and having –CH, – NH, and –SH functional groups were responsible for bioreduction and stabilization. In addition, the synthesized gold nanoparticles were found to be selectively bactericidal against the pathogenic gram negative bacteria, Escherichia coli. 4. Biogenic synthesis of floral-shaped gold nanoparticles using a novel strain, Talaromyces flavus Eepsita Priyadarshini • Nilotpala Pradhan • Lala Behari Sukla •Prasanna kumar Panda • Barada Kanta Mishra Annals of Microbiology September 2014, Volume 64, Issue 3, pp 1055-1063 ABSTRACT: A biogenic route was adopted towards the synthesis of gold nanoparticles using the extract of a novel strain, Talaromyces flavus . Reduction of chloroauric acid by the fungal extract resulted in the production of gold nanoparticle, which was further confirmed by the concordant results obtained from UV–visible spectroscopy, energy dispersive spectroscopy (EDS), and dynamic light scattering (DLS) analysis. Morphology and the crystal nature of the synthesized nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD) and selected area electron diffraction (SAED). A direct correlation was observed between nanoparticle formation and the concentration of reducing agent present in the fungal extract. The time-dependent kinetic study revealed that the bioreduction process follows an autocatalytic reaction. Crystalline, irregular, and mostly flowershaped gold nanoparticles with a mean hydrodynamic radius of 38.54±10.34 nm were obtained. pH played a significant role on production of mono-dispersed nanoparticle. FTIR analysis partially deciphered the involvement of –NH2, −SH, and –CO groups as the probable molecules in the bio-reduction and stabilization process. Compared to the conventional methods, a time-resolved, green, and economically viable method for floral-shaped nanoparticle synthesis was developed 5. BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING MICROALGA CHLOROCOCCUM HUMICOLA AND ITS ANTIBACTERIAL ACTIVITY Jayashree Jenaa • Nilotpala Pradhana • Bisnu Prasad Dashb • Lala Behari Sukla • Prasanna kumar Panda International Journal of Nanomaterials and Biostructures 2013; 3(1): 1-8 ABSTRACT: In the present investigation, synthesis of silver nanoparticles (AgNPs) using fresh extract and whole cell of microalga Chlorococcum humicola was carried out. The extract and the whole cell were incubated with AgNO3. The in-vivo and in- vitro formation of nanoparticles were characterized and investigated by Ultraviolet-Visible (UV-Vis) spectroscopy, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Fourier Transform Infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Bioreduction of algal extract showed a gradual change in the colour of the extract due to the formation of silver nanopaticles. The UV-Visible absorption spectrum recorded for the solution shows the distinguishing surface plasmon significance band for silver nanoparticles at 430nm. FT-IR analysis revealed the involvement of protein molecules in the formation of nanoparticles. TEM analysis confirms SNPs formed were 16 nm in size. Further the formed silver nanoparticles showed a strong antibacterial effect on the pathogenic bacteria Escherichia coli. So far, that test organism Chlorococcum humicola has not been reported to mediate nano material biosynthesis, this work confirms the microalga can be used as a suitable system for SNPs synthesis. The bioaccumulation of silver particles makes the organism potential candidate for ecofriendly silver biorecovery system in cost effective manner from industrial wastes. © 2013 Universal Research Publications. All rights reserved 6. In Situ Synthesis of Entrapped Silver Nanoparticles by a Fungus— Penicillium purpurogenum Pradhan, Nilotpala; Nayak, Rati Ranjan; Pradhan, Arun Kumar; Sukla, Lala Behari; Mishra, Barada Kanta, Nanoscience and Nanotechnology Letters, Volume 3, Number 5, October 2011, pp. 659-665(7) ABSTRACT : Intracellular silver nanoparticles are produced by exposing silver nitrate to a fungus—Penicillium purpurogenum. The formation of silver nanoparticles is greatly influenced by the secretion of proteins and more specifically secretion of nitrate reductase enzyme by the fungus. The UV-visible spectra show a broad band at 420 nm for spherical silver nanoparticles. The X-ray diffraction analysis indicates that the structure of the nanoparticles is face centered cubic. Results of transmission and scanning electron microscopy show that the silver nanoparticles are embedded within fungal mycelia. Finally, the biomass embedded nanoparticles are evaluated for their antimicrobial activity. They show significant antimicrobial activity against pathogenic gram negative bacteria likeEscherichia coli and Pseudomonas aeruginosa, and gram positive bacteria like Staphylococcus aureus. 7. Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: The process and optimization Rati Ranjan Nayak • Nilotpala Pradhan • Debadhyan Behera •Kshyama Madhusikta Pradhan • Srabani Mishra • Lala Behari Sukla • Barada Kanta Mishra, Journal of Nanoparticle Research August 2011, Volume 13, Issue 8, pp 3129-3137 ABSTRACT : An eco-friendly microbial method for synthesis of silver colloid solution with antimicrobial activity is developed using a fungal strain of Penicillium purpurogenum NPMF. It is observed that increase in concentration of AgNO3 increases the formation of silver nanoparticle. At 5 mM concentration highly populated polydispersed nanoparticles form. Furthermore, change in pH of the reaction mixture leads to change in shape and size of silver nanoparticles. At lower pH two peaks are observed in the absorption spectra showing polydispersity of nanoparticles. However, highly monodispersed spherical nanoparticles of 8–10 nm size form with 1 mM AgNO3 concentration at pH 8. Antimicrobial activity of nanoparticles is demonstrated against pathogenic gram negative bacteria like Escherichia coli and Pseudomonas aeruginosa, and gram positive bacteria like Staphylococcus aureus. The antimicrobial activity of silver nanoparticles obtained at different initial pH show strong dependence on the surface area and shape of the nanoparticles. 1.Homology modeling and docking studies of FabH ketoacyl-ACP synthase III) enzyme involved in typ II fatty acid biosynthesis of Chlorella variabilis: a potential algal feedstock for biofuel production,Namrata Misra , Mahesh Chandra Patra , Prasanna Kumar Panda Lala Bihari Sukla ,Barada Kanta Mishra ,Journal of Biomolecular Structure and Dynamics,iFirst, 2012, 1–17. 2.Low temperature oxygen plasma assisted surface modification of raw silk fibre and their characterizations,R. R. Nayak & L. B. Sukla & B. K. MishraInt ,J Plast Technol DOI 10.1007/s12588-013-9043-y 3..Biosynthesis and characterization of silver Nanoparticles using microalga chlorococcum humicola and its antibacterial activity,Jayashree Jena, Nilotpala Pradhana, Bisnu Prasad Dash, Lala Behari Sukla, Prasanna kumar Panda,International Journal of Nanomaterials and Biostructures 3(1),2013pp1-8. 4.In situ synthesis of entrapped silver nanoparticles by a fungus-Penicillium purpurogenum,Nilotpala Pradhan, Rati Ranjan Nayak, Arun Kumar Pradhan, Lala Behari Sukla and Barada Kanta Mishra,Journal of Nanoscience and Nanotechnology Letters,3 (5),2011,659-665.
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Institute for Applied Environmental Biotechnology Innovation,Bhubaneswar,www.utkalbiotech.org
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