Comments

• 

  Nagib Elmarzugi June 1, 2012 at 4:14am

  can some body email me a standard golden nanoparticle image please
• 

  TINC May 8, 2012 at 10:31pm

  Dear SPM-Producers,

   

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  Blogs, news services, forum topics, thematic groups will be focusing on SPMs during this six days.

   

  We invite SPM producers and ditributors to present they products to our members and visitors, coming from more than 80 countries http://www.nanopaprika.eu/profiles/members/

  Participating fee for comapnies is 990 USD which include:

   

  - 320x90 pixel banner on main page and all subpages of www.nanopaprika.eu for 1 year

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• 

  Ian Allen March 6, 2012 at 1:53pm

  In addition to the other advice already given:

  1) Reduce the physical size of your sample as small as possible.  More mass = more thermal vibrations.

  2) Reduce the temperature of your sample (and room) as much as feasible and comfortably possible.  

  3) Dont place your AFM on a table.  It should be mounted on a heavy slab which is in turn suspended by a system of springs, much like a DJ spinning board for techno music.  If you dont have such a setup, use the heaviest table you can find.

  4) If possible, use an adhesive to attach your sample to a holding chip, but use as little adhesive as possible.  Allow at least 24 hours after affixing with the adhesive before imaging, to relieve tension.

  5) If possible, load the AFM, focus, and then allow it to sit for several hours (or an entire day) in order to come to total thermal equilibrium and reduce thermal drift and vibrations.  
• 

  Dr.BAIJU G.NAIR May 26, 2010 at 12:03pm

  hi sujith
  its a good information.
  i will follow these things in future..
  i want to know more about cell sample observation...
• 

  Sujit Kumar May 26, 2010 at 11:48am

  Dear baiju,
  First of all look at the the mode of operation. If it is contact mode then try to use cantilevers having the lowest force constant. Tapping mode works the best for all biological samples. If you have problem during scanning u can always remove cantilever and put it in ethanol for few minutes before using it again.
  
  second thing that is important is to find a stable environment to avoid
  vibrations which can cause noise to any scanning probe micrscope. You can avoid this by closing the door of your room and asking everybody not to enter the room during measurements. Another important point is the electrical noise. So AFM power connection should be connected only to one socket and other instrument must not be connected in to that socket.
  
  Regarding during experiments try to play with your servo gain. too high or low value of servo gain can always result in noise.
  
  Hope this helps. If u have any specific problem then let me know.. These are just generalised problem associated with scanning probe microscopes.
  best wishes..
• 

  Dr.BAIJU G.NAIR May 26, 2010 at 10:53am

  hi sujith
  thanks for getting back to me..
  i am using asylum AFM
  one BIO-PD...
  not their latest saphire..
  regards
  bgnair
• 

  Sujit Kumar May 26, 2010 at 8:35am

  Hi Baiju,
  
  What mode of AFM are you using,,, could u eleborate ? Then only I will be able to comment about noise....
• 

  Dr.BAIJU G.NAIR October 31, 2009 at 5:43am

  Helo Researchers
  Is there any researchers who are specialized in AFM or SPM on biological sample.I am experiencing noises when i go down to a level of 200nm size scan.
  rgrds
• 

  Kuchana Srinivas August 9, 2009 at 5:35pm

  My instrument is CP II .
• 

  TINC May 9, 2009 at 10:48am

  

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