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  • Full member

    can some body email me a standard golden nanoparticle image please

  • Full member
    Dear SPM-Producers,
     
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  • Full member

    In addition to the other advice already given:

    1) Reduce the physical size of your sample as small as possible.  More mass = more thermal vibrations.

    2) Reduce the temperature of your sample (and room) as much as feasible and comfortably possible.  

    3) Dont place your AFM on a table.  It should be mounted on a heavy slab which is in turn suspended by a system of springs, much like a DJ spinning board for techno music.  If you dont have such a setup, use the heaviest table you can find.

    4) If possible, use an adhesive to attach your sample to a holding chip, but use as little adhesive as possible.  Allow at least 24 hours after affixing with the adhesive before imaging, to relieve tension.

    5) If possible, load the AFM, focus, and then allow it to sit for several hours (or an entire day) in order to come to total thermal equilibrium and reduce thermal drift and vibrations.  

  • Full member
    hi sujith
    its a good information.
    i will follow these things in future..
    i want to know more about cell sample observation...
  • Full member
    Dear baiju,
    First of all look at the the mode of operation. If it is contact mode then try to use cantilevers having the lowest force constant. Tapping mode works the best for all biological samples. If you have problem during scanning u can always remove cantilever and put it in ethanol for few minutes before using it again.

    second thing that is important is to find a stable environment to avoid
    vibrations which can cause noise to any scanning probe micrscope. You can avoid this by closing the door of your room and asking everybody not to enter the room during measurements. Another important point is the electrical noise. So AFM power connection should be connected only to one socket and other instrument must not be connected in to that socket.

    Regarding during experiments try to play with your servo gain. too high or low value of servo gain can always result in noise.

    Hope this helps. If u have any specific problem then let me know.. These are just generalised problem associated with scanning probe microscopes.
    best wishes..
  • Full member
    hi sujith
    thanks for getting back to me..
    i am using asylum AFM
    one BIO-PD...
    not their latest saphire..
    regards
    bgnair
  • Full member
    Hi Baiju,

    What mode of AFM are you using,,, could u eleborate ? Then only I will be able to comment about noise....
  • Full member
    Helo Researchers
    Is there any researchers who are specialized in AFM or SPM on biological sample.I am experiencing noises when i go down to a level of 200nm size scan.
    rgrds
  • Full member
    My instrument is CP II .
  • Full member

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