We are seeking highly qualified and motivated applicants with a Ph.D. at the time of employment. The post-doctoral position is funded by the French National Institute for Agricultural Research (INRA) for one year, possibly for two years if renewal is allowed, and is available immediately. Please if you are interested in this position reply to Cedric Gaillard at cedric.gaillard(aÖnantes.inra.fr . The candidate should
have a Ph.D. in chemistry or materials science or biology and mainly a strong background in scanning probe techniques and surface chemistry of AFM tips.
______________________________________
Description: AFM / Fluorescence correlative microscopy for the imaging of proteins at the interface of stabilized emulsions. Newly CEPIA research division funded post-doctoral position is available to investigate the building-up mechanisms of arrangement of proteins involved in the stabilisation of emulsions. The direct localisation of individual macromolecules remains an important challenge to elucidate their fine organisation at the nanometric scale and to connect it to the properties of the macroscopic systems formed by the assembly of the macromolecules. The complementarity of fluorescence detection and atomic force microscopy holds great promise for investigation of any systems
where the arrangement of biopolymers determines the macroscopic properties. In this project, combined AFM and fluorescence microscopy experiments will be performed to get the detection of two families of
proteins localized at the interface of emulsions. For that, a simple interface will be considered as a model in which a bovine protein and a vegetal protein are used for the stabilization of a water/oil emulsion.
Then, two parallel strategies of chemical modifications will been developed. Firstly, AFM tips will be functionalized with antibodies to allow specific AFM detection of the corresponding proteins at the
interface. Secondly, aqueous-soluble semiconductor nanocrystals (quantum dots) with specific size and fluorescence emission will be covalently bound to the proteins.
Our strategy that will be developed by the candidate is then based, in a third step, on the simulataneous detection by fluorescence of individual proteins, previously coupled with home- made quantum dots, and on AFM imaging using AFM cantilevers chemically-modified with pure and specific antibodies.
Nanomechanical properties of the individual proteins and of the corresponding antibodies will also be separatively probed by force spectroscopy to get information about the forces and kinetics involved
in the breaking of ligand – receptor bonds.

Dr. Cédric Gaillard
U.R. Biopolymčres Interactions Assemblages
INRA - BP 71 627
Rue de la Géraudičre
44 316 Nantes Cedex 3
cedric.gaillard(a)nantes.inra.fr

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Publications by A. Paszternák:

Smartphone-Based Extension of the Curcumin/Cellophane pH Sensing Method

Pd/Ni Synergestic Activity for Hydrogen Oxidation Reaction in Alkaline Conditions

The potential use of cellophane test strips for the quick determination of food colours

pH and CO2 Sensing by Curcumin-Coloured Cellophane Test Strip

Polymeric Honeycombs Decorated by Nickel Nanoparticles

Directed Deposition of Nickel Nanoparticles Using Self-Assembled Organic Template,

Organometallic deposition of ultrasmooth nanoscale Ni film,

Zigzag-shaped nickel nanowires via organometallic template-free route

Surface analytical characterization of passive iron surface modified by alkyl-phosphonic acid layers

Atomic Force Microscopy Studies of Alkyl-Phosphonate SAMs on Mica

Amorphous iron formation due to low energy heavy ion implantation in evaporated 57Fe thin films

Surface modification of passive iron by alkylphosphonic acid layers

Formation and structure of alkylphosphonic acid layers on passive iron

Structure of the nonionic surfactant triethoxy monooctylether C8E3 adsorbed at the free water surface, as seen from surface tension measurements and Monte Carlo simulations

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