BIOSYNTHESIS OF COBALT-COPPER NANOPARTICLES USING SENNA ALATA PLANT LEAF EXTRACT AND THEIR POTENTIAL THERAPEUTIC APPLICATIONS

Gangothri Kampa1, Sandhya Rani Edadhala1, Narasimha Golla1*
Department of Virology, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India.

Abstract

The present study aims to biogenically synthesize cobalt-copper (Co-Cu) nanocomposites from Senna alata leaf extract and to characterize them for therapeutic applications. Preliminary phytochemical screening of S. alata revealed the presence of carbohydrates, flavonoids, alkaloids, tannins, and phenolic chemicals, all of which play a crucial part in nanoparticle synthesis. These nanoparticles were characterized using scanning electron microscopy (SEM), UV-Visible spectroscopy, and Fourier-transform infrared spectroscopy (FTIR). The SEM revealed the spherical shape of the nanocomposites, UV-Visible exhibited a peak between 300 and 400nm, and FTIR analysis revealed that functional groups such as amino and carboxyl are responsible for the reduction and stabilization of nanoparticles. This study demonstrates that green synthesis using Senna alata leaf extract is a viable and eco-friendly approach for the formation of Cu & Co nanocomposite nanoparticles. These nanocomposites exhibit good multifunctional properties, including antibacterial activity against Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (E. Coli and Klebsiella) bacteria, and antifungal activity against the fungus A. niger. The free radical scavenging activities of Cu & Co nanocomposites were studied using DPPH and Hydrogen Peroxide in vitro assays, and their wound-healing activity was assessed by tissue regeneration. These activities make them promising candidates for biomedical applications.

Keywords: Biogenic fabrication, Cobalt-Copper nanocomposites, Senna alata, biomedical applications, and Wound healing.