Microscopic Insight into the death mechanism of single bacteria over of Graphene Oxide nanohybrid with KPFM
Sipajhar College, Darrang Assam
Abstract:
As we speak about hyegiene, the bacterial contamination has always been challenging. The attachments and the growth of the bacterial colony compromise the proper functioning of the surfaces which may lead to failure of equipments, like the eye lenses, prosthetic attachments, artificial bones, heart valves, dentures etc.. Food and baverages industries are also seen to be affected by bacterial contamination. Many research have been carried out extensively to search for alternatives for the antibacterial therapy. In this research, we have shown the composite of zinc-phthalocyanine-graphene oxide (ZnPc-GO) thin films as promising material for bactria growth inhibitors. With the help of Kelvin Probe Force Microscopy (KPFM) we could show that there is a temporal change of the bacterial cytoplasm during dying. KPFM helped in quantifying the mechanism of cell death by measuring (mapping) the transfer of charges at the interface of bacterial cell and composite surface due to the generated ROS-exhibited-stress. A time dependant study shows a clear picture of leakage of cytoplasm which contains the lipopolysaccharide that deals with bacterial surface potential. Since the charge transfer from bacterial cell happens to be an irreversible process, the cell dies and the charge spread in terms of cytoplasm leakage. In conclusion the microscopic single bacterial quantification of surface charge leakage may uncover the mechanism of bacteria death in depth.
Keywords: ZnPc-GO nanohybrid, KPFM, Anti-bacterial activity, surface potential
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