Gallic acid-mediated metallic nanoparticle synthesis. In vitro evaluation of cytotoxicity and radiosensitizing properties of the obtained nanomaterials

Bartosz Klębowski, Joanna Depciuch, Magdalena Parlińska-Wojtan

Institute of Nuclear Physics Polish Academy of Sciences,
Radzikowskiego 152, 31-342 Kraków



Recently, there have been trends regarding the use of green chemistry methods for the synthesis of nanomaterials. The application of such green methods is not only eco-friendly, but may also affect the biocompatibility of such nanoparticles in vitro. In the conducted research, gallic acid was used as a stabilizer and reducing agent in the synthesis of mono-, bi-and trimetallic nanoparticles based on gold, platinum and palladium. The morphology and microstructure of nanoparticles were assessed using scanning transmission electron microscopy (STEM), selected area electron diffraction (SAED) and X-ray diffraction (XRD). The cytotoxicity of pre-synthesized nanoparticles was checked on selected colon and glioma cell lines. Then, it was checked whether such nanoparticles could be used as radiosensitizers to increase the effectiveness of irradiation of cancer cells with a proton beam. A number of biological assays were used to evaluate the interaction of nanoparticles with living cells, such the colorimetric MTS test, annexin V-binding assay and clonogenic test. The obtained research results showed that simulated proton therapy supported by nanoparticles is more effective than monotherapy without the use of nanoparticles. Moreover, the effect also depends on the cell lines used - glioma cancer cells showed larger radiosensitivity compared to colorectal cancer cells. Moreover, the final effect of such therapy was not significantly influenced by the type of metal used, but rather by the morphology of the nanoparticles.

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