Application of biological waste materials in the biosynthesis of Silver nanoparticles
Jayanta Kumar Patra*, Gitishree Das
Research Institute of Integrative Life Sciences, Dongguk University-Seoul, Goyang-si, Republic of Korea
Silk is fundamentally made up of two types of proteins: fibroin (which covers 70-80% of the silk cocoon) and sericin (which covers around 20-30 %). Sericin, removed from the silk cocoon by the sericulture and textile industries in the process of making silk is a rich source of several bioactive compounds with numerous applications in the field of pharmaceutical, biomedical, cosmetic, and food industries. Bearing in mind, the massive potential of sericin-based materials, in the present investigation, an attempt has been made to extract sericin from the silk cocoon, purify it and use it in the synthesis of silver nanoparticles for its future applications in food, cosmetics, and biomedical fields. The sericin was extracted from the silk cocoon by the degumming method. The crude sericin solution at different dilutions was used for the synthesis of silver nanoparticles. After synthesis, the sericin-based silver nanoparticles were characterized using various characterization techniques such as UV-Vis spectroscopy, Transmission electron microscopy, energy-dispersive X-ray spectroscopy, dynamic light scattering zeta potential, and Fourier-transform infrared spectroscopy. After characterization, the synthesized NPs were subjected to various bio-potential studies such as antibacterial effects against a series of Gram-positive and Gram-negative foodborne pathogenic bacteria and antioxidant potentials.
Keywords: Silver nanoparticles, Silk cocoon; Sericin; antibacterial, antioxidant
Acknowledgment: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1G1A1004667), the Republic of Korea.