Antidiabetic, cytotoxicity and antioxidant activity of Pisum sativum outer peel aqueous extract mediated biosynthesized silver nanoparticles

Jayanta Kumar Patra1*, Gitishree Das1, Han-Seung Shin2

1 - Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.

2 - Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea

Abstract: Bioynthesis of silver nanoparticles using food waste materials and investigation of their biomedical applications has gathered substantial attention during recent times. In the current study, biosynthesis of silver nanoparticles (AgNPs), has been carried out by utilizing the aqueous extract of outer peel of Pisum sativum as the reducing agent followed by the investigation of its bio potential in terms of antidiabetic, cytotoxicity, and antioxidant activities. Characterization of AgNPs was done by UV-VIS spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and 464 nm for the samples exposed to direct sunlight, scanning electron microscopy and energy-dispersive X-ray analysis showed the surface nature and their elemental composition with a strong peak at 3 KeV that corresponded to Ag (61.85 wt %), Fourier-transform infrared spectroscopy predicted the functional groups involved, and X-ray powder diffraction showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10-25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase and cytotoxicity against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of DPPH at 100 µg/mL). In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments.



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