A low cost method of silver nanoparticles synthesis by Lavandula angustifolia, with antioxidant, antibacterial and antifungal activity
R. Șuică-Bunghez, R.M. Senin, M. Ganciarov, C.L. Nistor, M. Constantin, I. Raut, C. Firincă, R. Stoica
ICECHIM BUCHAREST ROMANIA
Nanotechnology refers to the science domain which often uses green and eco-friendly methods, beneficial to the environment. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. Herein, we developed a low cost technique for preparation of silver nanoparticles (AgNP) using ultrasound heating of AgNO3+plant extract solution. Lavandula angustifolia is one of the medicinal plant, with important benefits in homeopathic remedies for health and human body.
The first step of this study was to prepare a plant extract through different methods: ultrasound and magnetic agitation, in ethanol and hydroalcoholic mixture. The second step was to obtain silver nanoparticles and the third step was to characterise silver nanoparticles with this medicinal plant. The research detailed the antioxidant and antimicrobial activity of lavender extract and metallic nanoparticles formed. The formation of AgNP-plant extract, was conﬁrmed using ultraviolet visible spectroscopy (UV-VIS/Cintra 203) and Fourier transform infrared spectroscopy (FTIR/Perkin Elmer). The nanoparticles dimensions were confirmed by Dynamic light scattering (DLS/Nano SZ) technique. Antioxidant activity was made using DPPH assay and it were presented better results on AgNP-lavender than lavender extract. Antimicrobial activity was performed against Candida albicans, Escherichia coli and Staphylococcus aureus. The antibacterial and antifungal activity were evaluated by agar diffusion method against Gram-positive, Gram-negative bacteria and the yeast C. albicans. The results showed that the lavender flowers extract and metallic nanoparticles had the remarkable inhibition of the all tested microorganisms. Sample 1 was more effective against S. Aureus and C. albicans. The bacterium E coli was best inhibited by sample 3. In conclusion, the described experiments confirmed the efficacy of tested compounds as natural antimicrobials and antioxidants.