Effect of carbon nanoparticles on the viability of HL60 cells
Blanka Emődy-Kiss, János Fent and Susan Lakatos
Department of Pathophysiology, Laboratory Institute for Health Protection, Medical Centre of HDF
H-1134, Róbert Károly krt. 44., Budapest, Hungary
In the vast majority of human applications various carbon nanotubes enter the bloodstream. Apart from their original function, their direct effect on the cellular elements of the blood cannot be neglected. We have previously studied the effect of single-walled carbon nanotubes on platelets, mature granulocytes, and their complex formation. Each type of nanotubes may affect cells in different ways. In our present work, as a model, we have used the HL-60 human promyelocytic cell line that can be differentiated into granulocytes. We have examined the effects of the short and long single-walled as well as multi-walled carbon nanotubes and fullerene exerted on these cells. In our experience, regardless of the length of the nanotube, single-walled carbon nanotubes decrease while multi-walled counterparts do not affect the viability of HL-60 cells as detected by MTT tests. Similarly, fullerene does not affect cell viability.