Detection of Bovine Serum Albumin by hybrid structure based on carbon thin films

M.M. Abboud 1,2, E. A. Konshina 1, D.P. Shcherbinin 1 and V. V. Zakharov 1

1 ITMO University, Saint-Petersburg, Russia
2 Mansoura University, Mansoura, Egypt

Hydrogenated amorphous carbon (a-C:H) has received attenuation due to its wide application. The a-C:H films were obtained by chemical deposition of toluene vapor (CVD) in a glow discharge plasma at a direct current supported by a magnetron plasma localized near the anode. The soft and polymeric a-C:H films with strong light emission has been widely studied in many applications.
Bovine Serum Albumin (BSA) has numerous biological and biochemical applications necessary for the development of life science. BSA is composed of 582 amino acid residues with 17 cysteine cross-linked residues and 1 free cysteine, and its secondary structure is the most analogous to human serum albumin (HSA).
Owing to the numerous applications of BSA, several methods for detection of BSA were carried out depend on different materials such as metallic NPs, semiconductor quantum dots and carbon dots.
In our work, a-C:H thin films and their hybrid structure with silver NPs were used as based materials for detection of BSA depending on optical properties like absorbance and photoluminescence.

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