Biochemical and cellular characterization of novel components of MCS
Membrane contact sites (MCS), where two organelles come into very close proximity without fusing, are emerging as novel structures for inter-organelle communication. These sites of intimate contact generally form between the endoplasmic reticulum and a second organelle, including mitochondria and the plasma membrane.
We are using a combination of cellular and biochemical approaches including high-resolution light and electron microscopy, live cell imaging and optogenetic manipulation of proteins/lipids in vivo, to identify novel components of MCS and to study their functions at these sites.
We benefit from state-of-the-art proteomics and imaging facilities at IJM, including TIRF, spinning disk, confocal, high-resolution PALM/STORM and electron microscopy systems.
Applicants should have a background in cell biology and experience in molecular biology and biochemistry. Prior experience in live cell imaging and electron microscopy is favourable but not mandatory.