Title of the paper: Effect of biologically synthesized nanoparticles with plant products and chemotherapeutics against biofilm of clinical isolates of Staphylococcus aureus and Candida tropicalis

Authors: Karthick Raja Namasivayam Selvaraj, Elamathi Krishnamoorthi and Brijesh Kumar Singh

University/Institute: Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, India.

Abstracts: 
In the present study , the effect of synthesized silver nanoparticles together with plant products and commercially available drugs were evaluated against biofilm inhibition and its biochemical composition of clinical isolates of Candida tropicalis and Staphylococcus aureus . Silver nanoparticles were synthesized from Lactobacillus acidophilus 01 strain and tested with aqueous extracts of Aloe vera , ginger ( Zingiberofficinale ), garlic ( Allium sativum ), tulsi ( Ocimumtenuiflorum ), oils such as coconut oil and antifungal drugs fluconazole and itraconazole for C. tropicalis , and antibacterial antibiotics such as tetracycline, chloramphenicol for S. aureus. The nanoparticles were synthesized by dried biomass washing of Lactobacillus acidophilus 01 strain adopting standard conditions and the synthesized purified particles were characterized by UV - vis spectroscopy and scanning electron microscopy (SEM). The UV - vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical sil ver plasmon absorption maxima at 430 nm and SEM micrograph demonstrated uniform spherical particles with the size range of 45 – 60 nm. The energy dispersive X - ray spectroscopy (EDX) of the nanoparticles confirmed the presence of elemental silver signal as a strong peak. Synthesized silver nanoparticles, together with plant products and commercially available drugs, showed a maximum inhibitory effect against both the tested clinical isolates. In the case of C. tropicalis , themaximum inhibition was observed for silver nanoparticles with itraconazole, fluconazole and garlic, followed by silver nanoparticles with itraconazole, and garlic. Total carbohydrates and total proteins of the biofilm matrix were highly reduced in the respective treatments. In S. aureus ,sil ver nanoparticles with tetracycline, chloramphenicol and garlic recorded the maximum inhibition, followed by silver nanoparticles with chloramphenicol, and garlic. Distinct reduction in total carbohydrates and proteins of the biofilm matrix was also record ed in the respective treatments.

Links to the paper (link to the journal webpage): http://www.ijpijournals.com/Articles/PDF/163.pdf

link to the journal webpage: http://www.ijpijournals.com/journal.php?journal_id=5