Authors: Faiyazuddin, Md.; Ahmad, Niyaz; Iqbal, Zeenat; Talegaonkar, Sushma; Bhatnagar, Aseem; Krishen Khar, Roop; Jalees Ahmad, Farhan
1Nanomedicine Research Lab, Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), New Delhi 110062, India; 2Faculty of Pharmacy, Integral University, Lucknow 226060, Uttar Pradesh, India; 3Division of Nuclear Medicine, Institute of Nuclear Medicine & Allied Sciences (INMAS), Brig. S. K. Majumdar Road, Delhi-110054, India
Current Pharmaceutical Analysis, Volume 8, Number 2, May 2012, pp. 189-195 (7)
An ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometric method (UHPLC/ESI-Q-TOF-MS) for the analysis of terbutaline (TB) in Wistar rat plasma has been developed and validated. The chromatographic separation was achieved on a Waters ACQUITY UPLCTM BEH C18 (100.0 mmx2.1 mm; 1.7 μm) column using isocratic mobile phase, consisting of 2 mM ammonium acetate and acetonitrile (90: 10; v/v), at a flow rate of 0.25 mL min-1. The transitions occurred at m/z 226.19→152.12 for TB, and m/z 260.34→183.11 for the internal standard. The recovery of the analytes from Wistar rat plasma was optimized using liquid- liquid extraction technique (LLE) in ethyl acetate. The total run time was 3.0 min and the elution of TB occurred at 1.85±0.05 min. The linear dynamic range was established over the concentration range 1-1000 ng mL-1 (r2; 0.9938±0.0005) for TB. The intra-assay and inter-assay accuracy in terms of % CV was in between 1.8-3.5. The lower limit of quantitation (LLOQ) for TB was 1.0 ng mL-1. Analytes were stable under various conditions (in autosampler, during freeze-thaw, at room temperature, and under deepfreeze conditions). The developed method was successfully applied for pharmacokinetic profiling in rodents.