Raman microscopy is a widely used technique in pharmaceutical industry. It allows identifying and rapidly characterizing chemical compounds, functional groups, molecular conformers, authenticating various drugs. Integration of Raman spectroscopy with Atomic Force Microscopy opens a wide range of new capabilities in imaging and characterizing pharmaceutical products.
In this application note, the distribution of chemical components within Anadin tablet is studied. Special autofocus procedure using AFM feedback is implemented. As a result, chemical maps are obtained with high spatial resolution and free of any artifacts due to sample roughness. Correlation between sample chemical composition (from Raman maps) and grain structure (from AFM images) is studied.