Attend an Atomic Force Microscopy e-Seminar!

Agilent Technologies is proud to announce that we will be hosting two new AFM e-Seminars this October. These one-hour live events will be led by some of the most highly esteemed scientists in their respective fields. The unique Agilent e-Seminar format includes an open Q&A session in which all online attendees are welcome to query the presenters.

Thursday, 15 October

Time: 14:00 CET

First Topic: The Physics of TREC Imaging

Presenter: Dr. Peter Hinterdorfer (University of Linz)

Simultaneous topography and recognition imaging (TREC) allows the investigation of receptor distributions on natural biological surfaces under physiological conditions. Based on atomic force microscopy in combination with a cantilever tip carrying a ligand molecule, it enables simultaneous sensing of topography and recognition of receptor molecules with nanometer accuracy. Here, Dr. Hinterdorfer will introduce optimized handling conditions and physical properties of the cantilever-tip-sample ensemble, which are essential for proper interpretation of experimental data gained from this technique.

Second Topic: The 5 Best PicoTREC Papers

Presenter: Dr. Gerald Kada (Agilent)

Dr. Kada will present the basic Agilent PicoTREC technique for simultaneous topography and recognition imaging. He will then present excerpts and data from the five most recognized published papers associated with PicoTREC.

Tuesday, 27 October

Time: 14:00 CET

First Topic: Immobilization Techniques of Biological Samples for Aqueous AFM Imaging

Presenter: Dr. Andreas Ebner (University of Linz)

The immobilization of biological samples is a critical part of bio-AFM imaging. On one hand, the biological activity has to be kept intact; on the other hand, the fixation of the biological sample has to be sufficiently stable, last sufficiently long, and stay tight, while the roughness induced by the coupling should be kept to a minimum. Dr. Ebner will discuss several strategies, including simple electrostatic binding of proteins with positive net charge, use of bivalent ions to bind positively charged molecules like DNA, covalent binding of single proteins, artificial (e.g., S-layer and purple membrane) and native (e.g., membrane red blood cells and nuclear envelope).

Second Topic: Basic to Advanced Sample Preparation for Biological Imaging

Presenter: Dr. Gerald Kada (Agilent)

Proper preparation is a prelude to successful life science AFM imaging. Dr. Kada will discuss the basics of sample preparation for fixed- and live-cell imaging, cell membranes, proteins, and DNA. He will then discuss techniques for high-resolution imaging in buffer and the most common misconceptions concerning sample preparation.

Tuesday, 10 November

Time: 14:00 CET

Topic "Expanding Characterization of Materials with Kelvin Force Microscopy"

Presenter: Sergei Magonov, Ph.D. (Senior Research Scientist, Agilent)

In this seminar, Sergei Magonov will introduce high-resolution, single-pass Kelvin force microscopy. The comparative analysis of the amplitude and frequency detection of the electrostatic force response will be made. "Its applications to different materials (e.g., metals, semiconductors, and organic systems) will also be presented.

Join us for one or more of these exciting live events!


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