Antimicrobial activity of silver nanoparticles.

Replies

- - Idah > CH.L.R.Jaidev September 23, 2009 at 1:20pm
    
    According to the attached picture, in negative gram bacteria such as Escherichia, the lipid layers are more than in compare to the positive gram, Phospholipid, lipoprotein, and lipo poly saccharides(LPS), so I think the nano silver particles can pass through of this layers easier than in compare to positive gram bacteria and reach to protein. Also in negative gram, the Peptidoglycan layer is thinner, about 7-8 nanometer, than positive gram, about 20-80 nanometer, so the nano silver can reach to inner membrane easily. Mitochondria is in the inner membrane and there are some groups of protein in mitochondria that they are responsible to enzymes respiratory. As a result the nano silver can produce the free radical and cause to inactivation of these enzymes and finally to death of bacteria.
    ch2f6.jpg
    
    https://storage.ning.com/topology/rest/1.0/file/get/3440157046?profile=original
Browaeys September 23, 2009 at 8:15am

The NanoForum in Paris has organised this year 3 meetings on this topic.
All discussions are available there : http://www.vivagora.org/spip.php?rubrique56

The pb with nanosilver is the fact it is used everywhere. So we will face a threat in the environment and on useful bacterias we need in our wastes' epuration stations.
To know more you can get my book "Le Meilleur des nanomondes" (Brave new nanoworld) only in french

Dorothée Benoit Browaeys

http://www.libella.fr/buchet-chastel/index.php?post/2009/02/23/Le-M...

http://le-meilleur-des-nanomondes.blogspot.com/
- CH.L.R.Jaidev > Browaeys September 23, 2009 at 11:54am
  
  Dear Browaeys,
  Thank you for participating in the discussion and also thanks for offering me the book that you written "Le Meilleur des nanomondes". I will learn french language for reading your book. Thanks for your information.
Idah September 23, 2009 at 5:04am

I think nano silver particles can attach to cell wall firstly, and then, little by little, they can penetrate to cell and affect on thiol groups in protein and produce the free radical such as ROS,reactive oxygen species,and as a result inactive the respiratory enzymes and affect on DNA so death of bacteria .
- Amod Shepal > Idah September 25, 2009 at 7:18pm
  
  Hello,
  In case of Viruss e.g. HIV-I, its a site specific (gp 120 protein recepters) and size dependent binding mechanism that inhibits binding of virus to the host cell.
  
  Amod Shepal
  Microbiologist,
  Pune, India.
- CH.L.R.Jaidev > Idah September 23, 2009 at 11:55am
  
  dear farideh heidarpour,
  Your views are good and it may also a reason for the antimicrobial activity of the silver nanoparticle. Can you suggest any so that i can get through.
Manu Smriti Singh September 22, 2009 at 11:45pm

There are several articles regarding the same. Few are mentioned below-

Elchiguerra J L et al (2005), Interaction of Silver Nanoparticles with HIV-I, J Nanobiotechnology 3, 6.

Morones J R et al (2005) The Bactericidal Effect of Silver Nanoparticles, Nanotechnology 16, 2346 -2353.

Antimicrobial effects of silver nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, Volume 3, Issue 1, Pages 95-101.
- CH.L.R.Jaidev > Manu Smriti Singh September 23, 2009 at 11:58am
  
  Dear Manu smriti singh,
  Thanks for teh article references. I have the first two articles with me. If you have can you send me a copy of the third article?
  - Dr. Murugesan Subbiah > CH.L.R.Jaidev September 25, 2009 at 2:53am
    
    I thank u. Send ur article to mail address. I am also doing antimicrobial activities of silver nanoparticles using seaweed
    - Dr. Murugesan Subbiah > Dr. Murugesan Subbiah September 25, 2009 at 2:53am
      
      murugesan5@yahoo.com

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