Dr. Stephan Barcikowski's Videos (The International NanoScience Community) - The International NanoScience Community 2014-04-16T10:16:00Z http://www.nanopaprika.eu/video/video/listForContributor?screenName=lbzh23x3qnh6&rss=yes&xn_auth=no gold nanoparticle conjugation with charged peptide and DNA ligands - bivalent bioconjugate tag:www.nanopaprika.eu,2012-06-17:1612324:Video:181711 2012-06-17T20:09:49.439Z Dr. Stephan Barcikowski http://www.nanopaprika.eu/profile/DrStephanBarcikowski <a href="http://www.nanopaprika.eu/video/gold-nanoparticle-conjugation-with-charged-peptide-and-dna"><br /> <img alt="Thumbnail" height="180" src="http://api.ning.com:80/files/ICzn2XYc4Awl6YSgwucac9srilJYMN2h7rC5tSYxay6FrG8moQ-uhnUUlILE1*e2gw7*k9PqeYc4RQH2tTZ*KLZzObFKKe9G/833762219.jpeg?width=240&amp;height=180" width="240"></img><br /> </a> <br></br>Gold nanoparticles can easily be conjugated by more than one functional biomolecule, in particular if the nanoparticle is initially "naked" (ligand-free). Because of partial oxidation of gold surface atoms, the particle attracts electron donors, which adsorb and self-assemble. Any electron donative group works, but a terminal thiol group works best for… <a href="http://www.nanopaprika.eu/video/gold-nanoparticle-conjugation-with-charged-peptide-and-dna"><br /> <img src="http://api.ning.com:80/files/ICzn2XYc4Awl6YSgwucac9srilJYMN2h7rC5tSYxay6FrG8moQ-uhnUUlILE1*e2gw7*k9PqeYc4RQH2tTZ*KLZzObFKKe9G/833762219.jpeg?width=240&amp;height=180" width="240" height="180" alt="Thumbnail" /><br /> </a><br />Gold nanoparticles can easily be conjugated by more than one functional biomolecule, in particular if the nanoparticle is initially "naked" (ligand-free). Because of partial oxidation of gold surface atoms, the particle attracts electron donors, which adsorb and self-assemble. Any electron donative group works, but a terminal thiol group works best for anchoring on gold. Here, a cell penetrating peptide (positive backbone, animated green) and a targeting vector (nucleic acid, negative backbone, animated blue) are conjugated to the gold nanoparticle. This bivalent bioconjugate could be designed to penetrate into cells and target certain gene sequence of the chromosome inside the nucleus.<br /> ------: The projekt is supported by Deutsche Forschungsgemeinschaft (research see <a href="http://www.uni-due.de/barcikowski/forschen.htm">http://www.uni-due.de/barcikowski/forschen.htm</a>). functional nanoparticles in biotechnology - enabled by laser ablation in liquid biomolecule flow tag:www.nanopaprika.eu,2012-06-17:1612324:Video:181902 2012-06-17T20:05:43.455Z Dr. Stephan Barcikowski http://www.nanopaprika.eu/profile/DrStephanBarcikowski <a href="http://www.nanopaprika.eu/video/functional-nanoparticles-in-biotechnology-enabled-by-laser"><br /> <img alt="Thumbnail" height="180" src="http://api.ning.com:80/files/wSMhOpl3hT6BNAbBqjbJ1d4wwoK2d7eyixe5dWmd0iOhHDwU-K6aJZIq571srvMscKzktE2ljwdNWaJBo6S67pYA6AY4FAv5/900003731.jpeg?width=240&amp;height=180" width="240"></img><br /> </a> <br></br>Functional nanoparticles may help to target and sort bovine cells. In this animation, the cell membrane is encountered by a "Trojan horse" made of a cationic cell penetrating peptide and a gene which both are anchored to a gold nanoparticle carrier that brings the bioconjugate to the site inside the cell nucleus. Both molecules require different media… <a href="http://www.nanopaprika.eu/video/functional-nanoparticles-in-biotechnology-enabled-by-laser"><br /> <img src="http://api.ning.com:80/files/wSMhOpl3hT6BNAbBqjbJ1d4wwoK2d7eyixe5dWmd0iOhHDwU-K6aJZIq571srvMscKzktE2ljwdNWaJBo6S67pYA6AY4FAv5/900003731.jpeg?width=240&amp;height=180" width="240" height="180" alt="Thumbnail" /><br /> </a><br />Functional nanoparticles may help to target and sort bovine cells. In this animation, the cell membrane is encountered by a "Trojan horse" made of a cationic cell penetrating peptide and a gene which both are anchored to a gold nanoparticle carrier that brings the bioconjugate to the site inside the cell nucleus. Both molecules require different media and pH, so it would be difficult to simply mix everything. But subsequent addition of the biomolecules to a continuous gold nanoparticle source (with its origin from laser ablation of a wire) flow in water could provide the bivalent gold nanoparticle bioconjugates.<br /> -----: The projekt is supported by Deutsche Forschungsgemeinschaft (research see <a href="http://www.uni-due.de/barcikowski/forschen.htm">http://www.uni-due.de/barcikowski/forschen.htm</a>). gold nanoparticle formation during laser ablation in liquid - shock wave cavitation nucleation tag:www.nanopaprika.eu,2012-06-17:1612324:Video:181621 2012-06-17T20:02:07.428Z Dr. Stephan Barcikowski http://www.nanopaprika.eu/profile/DrStephanBarcikowski <a href="http://www.nanopaprika.eu/video/gold-nanoparticle-formation-during-laser-ablation-in-liquid-shock"><br /> <img alt="Thumbnail" height="180" src="http://api.ning.com:80/files/sY2BV21gORf9ii4phhY4ZscuHPm9-JCJbLJt0dfJV-P1UnmAuxpx5VlBvi9H7*ZDBF2TtXEuo2LpNzxsg-HIEqGK6NFyEHcI/429308439.jpeg?width=240&amp;height=180" width="240"></img><br /> </a> <br></br>This animation simplifies the cascade of events happening during pulsed laser ablation in liquid. In detail, pulsed laser ablation of a thin wire in water… <a href="http://www.nanopaprika.eu/video/gold-nanoparticle-formation-during-laser-ablation-in-liquid-shock"><br /> <img src="http://api.ning.com:80/files/sY2BV21gORf9ii4phhY4ZscuHPm9-JCJbLJt0dfJV-P1UnmAuxpx5VlBvi9H7*ZDBF2TtXEuo2LpNzxsg-HIEqGK6NFyEHcI/429308439.jpeg?width=240&amp;height=180" width="240" height="180" alt="Thumbnail" /><br /> </a><br />This animation simplifies the cascade of events happening during pulsed laser ablation in liquid. In detail, pulsed laser ablation of a thin wire in water ca... clean nanoparticle colloids for medical and catalysis application tag:www.nanopaprika.eu,2012-02-06:1612324:Video:167555 2012-02-06T15:15:37.417Z Dr. Stephan Barcikowski http://www.nanopaprika.eu/profile/DrStephanBarcikowski <a href="http://www.nanopaprika.eu/video/clean-nanoparticle-colloids-for-medical-and-catalysis-application"><br /> <img alt="Thumbnail" height="180" src="http://api.ning.com:80/files/Zx2GNpAjY-DPdjfvBIHX8YSUrebIgdNOSe993hXFiFGj9i0NwnZkctTsmI8QQWSthx8S4lqBt9jLSmiEunf8ggYrdNkw0pm5/493145114.jpeg?width=240&amp;height=180" width="240"></img><br /> </a> <br></br>"Naked" nanoparticles for medical application and catalyst surfaces which require high purity: Luckily, the nanoparticles made physically by laser ablation are free of residual chemicals (free of ligands) that may cause unwanted cross-effects or blocking of the… <a href="http://www.nanopaprika.eu/video/clean-nanoparticle-colloids-for-medical-and-catalysis-application"><br /> <img src="http://api.ning.com:80/files/Zx2GNpAjY-DPdjfvBIHX8YSUrebIgdNOSe993hXFiFGj9i0NwnZkctTsmI8QQWSthx8S4lqBt9jLSmiEunf8ggYrdNkw0pm5/493145114.jpeg?width=240&amp;height=180" width="240" height="180" alt="Thumbnail" /><br /> </a><br />"Naked" nanoparticles for medical application and catalyst surfaces which require high purity: Luckily, the nanoparticles made physically by laser ablation are free of residual chemicals (free of ligands) that may cause unwanted cross-effects or blocking of the surface. nanoparticle agglomerate size reduction and fragmentation by laser excitation tag:www.nanopaprika.eu,2012-02-06:1612324:Video:167553 2012-02-06T15:14:02.523Z Dr. Stephan Barcikowski http://www.nanopaprika.eu/profile/DrStephanBarcikowski <a href="http://www.nanopaprika.eu/video/nanoparticle-agglomerate-size-reduction-and-fragmentation-by"><br /> <img alt="Thumbnail" height="180" src="http://api.ning.com:80/files/S6rBZjZXQE-b8udVaoCnWMs7mcZZjuast0oA5OcDAmNYELK-8axCPP8PLefx-sfgOSeajTQOcBWBd3RfrbRU5bT4SHIH5lz2/967505285.jpeg?width=240&amp;height=180" width="240"></img><br /> </a> <br></br>lasers can be used to reduce the size of nanoparticle colloids. the real-time video shows two vessels with agglomerated (blue colour) gold nanoparticle… <a href="http://www.nanopaprika.eu/video/nanoparticle-agglomerate-size-reduction-and-fragmentation-by"><br /> <img src="http://api.ning.com:80/files/S6rBZjZXQE-b8udVaoCnWMs7mcZZjuast0oA5OcDAmNYELK-8axCPP8PLefx-sfgOSeajTQOcBWBd3RfrbRU5bT4SHIH5lz2/967505285.jpeg?width=240&amp;height=180" width="240" height="180" alt="Thumbnail" /><br /> </a><br />lasers can be used to reduce the size of nanoparticle colloids. the real-time video shows two vessels with agglomerated (blue colour) gold nanoparticle collo...