gold nanoparticle conjugation with charged peptide and DNA ligands - bivalent bioconjugate

Gold nanoparticles can easily be conjugated by more than one functional biomolecule, in particular if the nanoparticle is initially "naked" (ligand-free). Because of partial oxidation of gold surface atoms, the particle attracts electron donors, which adsorb and self-assemble. Any electron donative group works, but a terminal thiol group works best for anchoring on gold. Here, a cell penetrating peptide (positive backbone, animated green) and a targeting vector (nucleic acid, negative backbone, animated blue) are conjugated to the gold nanoparticle. This bivalent bioconjugate could be designed to penetrate into cells and target certain gene sequence of the chromosome inside the nucleus.
------: The projekt is supported by Deutsche Forschungsgemeinschaft (research see http://www.uni-due.de/barcikowski/forschen.htm).

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Comment by sami ul haq on January 15, 2013 at 8:39pm

dear sir

we have synthsys the mg doped zno our particles size decrease ,please explane it ,


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Comment by Dr. Md. Faiyazuddin on September 4, 2012 at 10:32am

i want to make gold nanoconjugate with Unani drug. Kindly help me.


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Comment by Dr. Stephan Barcikowski on June 21, 2012 at 9:40am

dear Wale,

yes, of course. The protocol was published in an Open Access Journal, and there is another detail on the langth of the biomolecule spacer that you would have to consider for optimal conjugation efficiency.

Here the two references:

Barchanski, A. ; Sajti, C. L. ; Sehring, C. ; Petersen, S. ; BARCIKOWSKI, S.: Design of Bi-functional Bioconjugated Gold Nanoparticles by Pulsed Laser Ablation with Minimized Degradation. In: JLMN - Journal of Laser Micro/Nanoengineering 6 (2011), Nr. 2, S. 124–130
Impact of Spacer and Strand Length on Oligonucleotide Conjugation to the Surface of Ligand-Free Laser-Generated Gold Nanoparticles

http://pubs.acs.org/doi/abs/10.1021/bc200462b


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Comment by OYEDIRAN OYEWALE AKANFE on June 20, 2012 at 8:07pm

This is very briliant, Dr. stephan, will you be kind enough to share the protocol of how you synthesis this in the Lab with me? thank you

wale

Nigeria


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Comment by Dr. Stephan Barcikowski on June 18, 2012 at 9:50am

Dear Navin Jain,

it is an Animation based on experimental results (Open access Publication: Barchanski, A. ; Sajti, C. L. ; Sehring, C. ; Petersen, S. ; BARCIKOWSKI, S.: Design of Bi-functional Bioconjugated Gold Nanoparticles by Pulsed Laser Ablation with Minimized Degradation. In: JLMN - Journal of Laser Micro/Nanoengineering 6 (2011), Nr. 2, S. 124–130)

The animation software was run by Martin Langhammer (http://www.youtube.com/user/FRONTSICHT).


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Comment by Navin Jain on June 18, 2012 at 5:21am

First of all, congrats for such a nice approach.

Is this study also includes in silico experiments??

Can you share the details of the software used for preparing the video???

Thanks in advance

Navin Jain


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Comment by Dr. Stephan Barcikowski on June 17, 2012 at 10:12pm

Often, only one function is not enough. Multivalent gold nanoparticle conjugates combine two functions. Here: the cationic peptide helps translocating the cell membrane and the oligonucleotide allows binding to gene sequence inside the nucleus. 

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