functional nanoparticles in biotechnology - enabled by laser ablation in liquid biomolecule flow

Functional nanoparticles may help to target and sort bovine cells. In this animation, the cell membrane is encountered by a "Trojan horse" made of a cationic cell penetrating peptide and a gene which both are anchored to a gold nanoparticle carrier that brings the bioconjugate to the site inside the cell nucleus. Both molecules require different media and pH, so it would be difficult to simply mix everything. But subsequent addition of the biomolecules to a continuous gold nanoparticle source (with its origin from laser ablation of a wire) flow in water could provide the bivalent gold nanoparticle bioconjugates.
-----: The projekt is supported by Deutsche Forschungsgemeinschaft (research see

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Comment by G. Dharanivasan on June 21, 2012 at 9:58am

Thank you for reply. is this method is time consuming and cost effective?

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Comment by Dr. Stephan Barcikowski on June 21, 2012 at 9:49am

Dear Colleagues,

thanks for the feedback and questions!

yes, silver can be fabricated as well. Hovever, as you know, silver slowely dissolutes in water and thiol bond (which is the anchor between nanoparticle and the biomolecule) is weaker compared to gold.

We never tried EC separation. For validation of this method (whoich is still under development, the video is just an animation of the project outline), we fully focus on  reproduction biology.



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Comment by G. Dharanivasan on June 21, 2012 at 8:07am

simple and novel technology to synthesis but can be synthesis silver nanoparticles using this method?

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Comment by Sumit Pramanik on June 21, 2012 at 5:59am

It is very handy and novel technique. Can you detect and separate out bovine endothalial cells from its mixture of fibroblast cells using this technique?

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Comment by Dr. Vandna Luthra on June 21, 2012 at 2:57am

Very nice.


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Comment by Dr. Stephan Barcikowski on June 17, 2012 at 10:08pm

Nanoparticles, Biomolecules and Cells: This animation tries to summarize a current project on the design of bioconjugated nanoparticles targeting farm animal sperm cells.

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