Our group has successfully used electron microscopy to visualize the three-dimensional (3D) morphology of nascent autophagosomes. We have demonstrated that nascent autophagosomes can have membrane connections with several other organelles, including endoplasmic reticulum, mitochondria, lipid droplets, Golgi complex and endosomes/lysosomes (Ylä-Anttila et al. 2009, Biazik et al. 2015). The project aims to continue this research by combining live-cell imaging, correlative light-electron microscopy, cryofixation, freeze substitution and 3D electron microscopy. A successful candidate should have previous experience on biological electron microscopy and an interest in cell morphology.
The research will take place at the University of Helsinki, Department of Biosciences, located on the Viikki campus 10 km North from the center of Helsinki. The campus has excellent core facilities for light and electron microscopy. The project will include a research visit to the laboratory of our collaborator at the University of Tokyo, Japan.
More information on the supervisor of the project: http://tuhat.halvi.helsinki.fi/portal/en/person/eleskeli
Additional information and applications::
Email: eeva-liisa.eskelinen-at-helsinki.fi
Deadline for applications is September 30, 2015.
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