Microbicidal tissue paper is made by a simple method of impregnating biosynthesized silver nanoparticles with the conventional tissue paper. A number of infections are being transmitted by conventional tissue paper especially through hospitals. Silver nanoparticles were synthesized by green synthetic route using the leaf extract of Eichornia crassipes, further characterized using UV Vis spectroscopy exhibiting a SPR peak at 413 nm and HRTEM having a size between 20 to 50 nm. We have developed a hybrid tissue paper for control of spreading of hospital infections. The hybrid tissue paper was characterized by X – Ray Diffraction and Field Emission Scanning Electron Microscopy in which the size of the nanoparticles present in the tissue paper was found to be ranging from 20 nm to 50 nm. Antimicrobial activity was evaluated against clinical pathogens - Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and release studies of nanoparticles from the hybrid tissue paper were carried out to ascertain the antimicrobial nature.

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