Producing the Controlled Hybrid Lattice of Natural Protein Nanotubes by Chemical Hydrolysis for Nanomedicine

Authors: Pegah Esmaeilzadeh, Zahra Fakhroueian, Pouriya Esmaeilzadeh

Abstract: This work was instigated by the fact that sol-gel chemistry provides a relatively simple way to incorporate recognition species in a stable host green environment. In this new strategy, which represents a low cost example of bottom-up nano-assembly, chemistry art is entering the field of nanobiotechnology in fabrication and control of an expanded homogeneous length of separate single-walled and ordered, helical lattice-like open-ended natural protein nanotubes(PNTs) and also creative novel bio-nanohybrids. A new type of protein nanohybrids containg nanotubes-nanorods, nanotubes-nanofibers, nanotubes core-shell nanofibers were prepared using an electrostatic self-assembly method with the aid of chemical partial hydrolysis of milk protein α-lactalbumin (sol-gel technique) at a suitable pH value for the first time. They can have long helically coiled length and are promising for high capacity drug loading and applying in nanomedicine as organ transplantation in human body and implant material, because of their improved stability and unique mechanical and lattice thermal resistivity properties. In this study, various valuable ligand or binding sites such as distinct Mn⁺², Ca⁺²or Zn⁺² cations were used for incorporated into protein nanostructures as the self-assembly essential stimulant motor. It was found that the designed nanobioproducts could retain and stabilize as very clear and transparent green aqueous nanobiofluids during two years.

Direct link: http://www.scientific.net/JNanoR.21.7