Conventional and Nanotechniques for DNA Methylation Profiling

DNA methylation is critical for gene silencing and is associated with the incidence of many diseases,

including cancer. Underlying molecular mechanisms of human diseases and tissue-specific gene

expression have been elucidated based on DNA methylation studies. This review highlights the

advantages and drawbacks of various methylation screening techniques: blotting, genomic sequencing,

bisulfite sequencing, methylation-specific PCR, methylated DNA immunoprecipitation, microarray

analysis, matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, nanowire transistor

detection procedure, quantum dot-based nanoassay, single-molecule real-time detection,

fluorimetric assay, electrochemical detection, and atomic force spectroscopy. The review provides insight

for selecting a method or a combination of methods for DNA methylation analysis. Convergence of

conventional and contemporary nanotechniques to enumerate methylation at speci

fic CpG sites of oncogene would fill the gap in diagnosis of cancer. (J Mol Diagn 2013, 15: 17e26; http://dx.doi.org/

10.1016/j.jmoldx.2012.06.007DNA methylation)

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Welcome! Nanopaprika was cooked up by Hungarian chemistry PhD student in 2007. The main idea was to create something more personal than the other nano networks already on the Internet. Community is open to everyone from post-doctorial researchers and professors to students everywhere.

There is only one important assumption: you have to be interested in nano!

Nanopaprika is always looking for new partners, if you have any idea, contact me at editor@nanopaprika.eu

Dr. András Paszternák, founder of Nanopaprika

Publications by A. Paszternák:

The potential use of cellophane test strips for the quick determination of food colours

pH and CO2 Sensing by Curcumin-Coloured Cellophane Test Strip

Polymeric Honeycombs Decorated by Nickel Nanoparticles

Directed Deposition of Nickel Nanoparticles Using Self-Assembled Organic Template,

Organometallic deposition of ultrasmooth nanoscale Ni film,

Zigzag-shaped nickel nanowires via organometallic template-free route

Surface analytical characterization of passive iron surface modified by alkyl-phosphonic acid layers

Atomic Force Microscopy Studies of Alkyl-Phosphonate SAMs on Mica

Amorphous iron formation due to low energy heavy ion implantation in evaporated 57Fe thin films

Surface modification of passive iron by alkylphosphonic acid layers

Formation and structure of alkylphosphonic acid layers on passive iron

Structure of the nonionic surfactant triethoxy monooctylether C8E3 adsorbed at the free water surface, as seen from surface tension measurements and Monte Carlo simulations

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