Collagen Fibers3

Collagen Fibers3

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  • Dear Prerna
    WOZNIAK.Michaljerzy@nims.go.jp
    Regards
    Michal
  • Dear Michal,
    Thanks for the reply. i wanted to show my TEM image of recombinant collagen like triple helix, can you give your email ID
  • Dear Prerna
    The material was porcine type I collagen on Poly(DL-lactic-co-glycolic acid) (PLGA) freeze dried.
    Details:
    A PLGA film was prepared by casting PLGA solution in chloroform at a concentration of 27 (w/v) % on cover glass (diameter, 18 mm), and then annealed at 60C for 24h. The PLGA films were immersed in type I collagen acidic solution (0.5 %, pH 3) under reduced pressure, centrifuged at 1200 G for 10 min at 4C, and then vacuum-dried or freeze-dried.
    In fact this sample was unusual, something went wrong in the process, typically we get flat samples after preparation in such a way. But there is nice image.

    Regards
    Michal
  • could you please explain this pic of collagen fibers? like it this the AFM image? what type of collagen is this? how did you prepare the sample.

    i have the TEM picture of the collagen, its very similar to yours, i need some info, could you reply ASAP
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