Scanning Probe Microscopy


Scanning Probe Microscopy

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Latest Activity: Nov 15, 2012

Discussion Forum


Started by Gyanesh Singh. Last reply by Alexander Yalovenko Nov 15, 2012. 3 Replies

NT-MDT users

Started by TINC Feb 2, 2008. 0 Replies

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Comment by Nagib Elmarzugi on June 1, 2012 at 4:14am

can some body email me a standard golden nanoparticle image please

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Comment by TINC on May 8, 2012 at 10:31pm
Dear SPM-Producers,
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Blogs, news services, forum topics, thematic groups will be focusing on SPMs during this six days.
We invite SPM producers and ditributors to present they products to our members and visitors, coming from more than 80 countries
Participating fee for comapnies is 990 USD which include:
- 320x90 pixel banner on main page and all subpages of for 1 year
- 5 blogpost about products of the company (based on the materials send by the company) between 20-25th May 2012
- 1 introducing  direct mail for 5550 members (text or simple html, maximum 8000 characters)
- 320x90 pixel banner and short info text placing on events page /will be live between 20-25th May 2012/

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Comment by Ian Allen on March 6, 2012 at 1:53pm

In addition to the other advice already given:

1) Reduce the physical size of your sample as small as possible.  More mass = more thermal vibrations.

2) Reduce the temperature of your sample (and room) as much as feasible and comfortably possible.  

3) Dont place your AFM on a table.  It should be mounted on a heavy slab which is in turn suspended by a system of springs, much like a DJ spinning board for techno music.  If you dont have such a setup, use the heaviest table you can find.

4) If possible, use an adhesive to attach your sample to a holding chip, but use as little adhesive as possible.  Allow at least 24 hours after affixing with the adhesive before imaging, to relieve tension.

5) If possible, load the AFM, focus, and then allow it to sit for several hours (or an entire day) in order to come to total thermal equilibrium and reduce thermal drift and vibrations.  

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Comment by BAIJU G.NAIR on May 26, 2010 at 12:03pm
hi sujith
its a good information.
i will follow these things in future..
i want to know more about cell sample observation...

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Comment by Sujit Kumar on May 26, 2010 at 11:48am
Dear baiju,
First of all look at the the mode of operation. If it is contact mode then try to use cantilevers having the lowest force constant. Tapping mode works the best for all biological samples. If you have problem during scanning u can always remove cantilever and put it in ethanol for few minutes before using it again.

second thing that is important is to find a stable environment to avoid
vibrations which can cause noise to any scanning probe micrscope. You can avoid this by closing the door of your room and asking everybody not to enter the room during measurements. Another important point is the electrical noise. So AFM power connection should be connected only to one socket and other instrument must not be connected in to that socket.

Regarding during experiments try to play with your servo gain. too high or low value of servo gain can always result in noise.

Hope this helps. If u have any specific problem then let me know.. These are just generalised problem associated with scanning probe microscopes.
best wishes..

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Comment by BAIJU G.NAIR on May 26, 2010 at 10:53am
hi sujith
thanks for getting back to me..
i am using asylum AFM
one BIO-PD...
not their latest saphire..

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Comment by Sujit Kumar on May 26, 2010 at 8:35am
Hi Baiju,

What mode of AFM are you using,,, could u eleborate ? Then only I will be able to comment about noise....

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Comment by BAIJU G.NAIR on October 31, 2009 at 5:43am
Helo Researchers
Is there any researchers who are specialized in AFM or SPM on biological sample.I am experiencing noises when i go down to a level of 200nm size scan.

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Comment by Kuchana Srinivas on August 9, 2009 at 5:35pm
My instrument is CP II .

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Comment by TINC on May 9, 2009 at 10:48am

Free Online AFM Seminars

Agilent Technologies is proud to announce a brand new series of AFM e-Seminars this spring! These one-hour live events will be led by some of the most prestigious scientists in their respective fields. The unique Agilent e-Seminar format includes an open Q&A session in which all online attendees are welcome to query the presenters.

Choosing the Correct Cantilever for your Application
May 13, 2009 at 3PM GMT

Speakers: Oliver Krause, PhD NanoWorld Group
Probe Manufacturing - Why Probes Look the Way They Do.

In this seminar, Dr Krause will explain the reason why probes have many shapes, the different probe types that are available and what extra refinements can be done for various applications. This seminar will also give you a guide line of how to choose the correct probe for your application

Speaker: Arvind Raman, PhD Purdue University
Veda: a Free Online Simulation Tool for the AFM Community

VEDA (Virtual environment for dynamic AFM) is a suite of free, cyber-enabled tools available on that simulate tapping mode AFM using magnetic/acoustic excitation (MAC/AAC) on organic and inorganic surfaces in liquids as well as ambient conditions. In this seminar, a brief introduction to the tools and their use in choosing cantilevers and operating conditions for optimal imaging will be presented


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