Comment

Comment by BAIJU G.NAIR on May 26, 2010 at 12:03pm

hi sujith
its a good information.
i will follow these things in future..
i want to know more about cell sample observation...

Comment by Sujit Kumar on May 26, 2010 at 11:48am

Dear baiju,
First of all look at the the mode of operation. If it is contact mode then try to use cantilevers having the lowest force constant. Tapping mode works the best for all biological samples. If you have problem during scanning u can always remove cantilever and put it in ethanol for few minutes before using it again.

second thing that is important is to find a stable environment to avoid
vibrations which can cause noise to any scanning probe micrscope. You can avoid this by closing the door of your room and asking everybody not to enter the room during measurements. Another important point is the electrical noise. So AFM power connection should be connected only to one socket and other instrument must not be connected in to that socket.

Regarding during experiments try to play with your servo gain. too high or low value of servo gain can always result in noise.

Hope this helps. If u have any specific problem then let me know.. These are just generalised problem associated with scanning probe microscopes.
best wishes..

Comment by BAIJU G.NAIR on May 26, 2010 at 10:53am

hi sujith
thanks for getting back to me..
i am using asylum AFM
one BIO-PD...
not their latest saphire..
regards
bgnair

Comment by Sujit Kumar on May 26, 2010 at 8:35am

Hi Baiju,

What mode of AFM are you using,,, could u eleborate ? Then only I will be able to comment about noise....

Comment by BAIJU G.NAIR on October 31, 2009 at 5:43am

Helo Researchers
Is there any researchers who are specialized in AFM or SPM on biological sample.I am experiencing noises when i go down to a level of 200nm size scan.
rgrds

Comment by Kuchana Srinivas on August 9, 2009 at 5:35pm

My instrument is CP II .

Comment by TINC on May 9, 2009 at 10:48am

Free Online AFM Seminars

Agilent Technologies is proud to announce a brand new series of AFM e-Seminars this spring! These one-hour live events will be led by some of the most prestigious scientists in their respective fields. The unique Agilent e-Seminar format includes an open Q&A session in which all online attendees are welcome to query the presenters.

Choosing the Correct Cantilever for your Application
May 13, 2009 at 3PM GMT

Speakers: Oliver Krause, PhD NanoWorld Group
Probe Manufacturing - Why Probes Look the Way They Do.

In this seminar, Dr Krause will explain the reason why probes have many shapes, the different probe types that are available and what extra refinements can be done for various applications. This seminar will also give you a guide line of how to choose the correct probe for your application

Speaker: Arvind Raman, PhD Purdue University
Veda: a Free Online Simulation Tool for the AFM Community

VEDA (Virtual environment for dynamic AFM) is a suite of free, cyber-enabled tools available on www.nanohub.org that simulate tapping mode AFM using magnetic/acoustic excitation (MAC/AAC) on organic and inorganic surfaces in liquids as well as ambient conditions. In this seminar, a brief introduction to the tools and their use in choosing cantilevers and operating conditions for optimal imaging will be presented

Register

Comment by Andrei Kholkin on March 22, 2009 at 4:49pm

Dear All:
We will soon host the 4th Piezoresponse Force Microsocpy workshop and a satellite Symposium on Nanoscale Phenomena in Polar Materials (PFM 2009). Please, see details in pfm4.web.ua.pt.
Please, join us to learn how nanoscale piezoelectricity works.
Andrei Kholkin

Comment by Brad Rangell on March 3, 2009 at 5:59am

This is a link to an abstract on scanning ion conductance microscopy recently published in Nature Methods. http://www.nature.com/nmeth/index.html. If you are interested in high resolution live cell imaging you should take a minute to check it out.

Comment by zeynep meric on January 3, 2009 at 11:59am

hello everyone,
Can ı send my images from my lab and images that ı got from my experiments to this competition?

• ‹ Previous
• 1
• 2
• Next ›
• Page