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How do we find out the biosynthesis of silver nanoparticles

How do we find out the bio synthesis of silver nanoparticles taking place in the organisms,whether it is intracellular or extracellular and what are the pathways which are leading to the bio synthesis of silver nanoparticles.

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I guess there is no specific mechanism that aids in the biosynthesis of silver nano particle.

In my previous research i found that one marine fungi didnt produce extra cellular fungi but when the filtrate was subjected to sonication, it gave promising results.

whereas few soil- derived fungi produced Ag nano particles extracellularly.

I feel that the exact mechanism and process remains skeptical
Reducing monosacharides in the intra or extra cellular fluids can reduce Ag+ ions into Ag nano particles
Thank U for the info Mr Kondalarao

Hope the given link and the article will give an idea about the intracellular or extracellular biosynthesis of nanoparticles..

http://www.iqm.unicamp.br/graduacao/material/qf933/nayaran-revie-bi...
Thank u Mr Inbakandan for the article i will go through it but i do also have similar articles on my side.
hey that was a useful reference ... thanks it helped
Hi Vishnu - The most environmental friendly and the best route to large-scale production of silver nanoparticles is reported in this patent: www.faqs.org/patents/app/20100055199
Ideal for capital veturists!
Dear Asher,

Thank u so much for the info.

Regards
vishnu
every were ppl talk abt producing silver nano particle from fungi or algae as a potent source than bacterial usage ... is it so ?

biosynthesis of silver nanopartilces by living source is the topical research. it is not the matter of fungi or algae or bacteria, it is all depends on the capping or reducing agent which triggers the reduction of silver precursors to nanosilver. literature says that the unique physio-chemical properties also varies according to the capping or reducing agent.

i m using marine sponges as living source and synthesizing silver nanoparticles for marine biofouling control or antifouling applications.

 

well i am using marine algae
brown color changes + UV- Spectrophotometer absorption peak (420)

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