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Permalink Reply by Idah on September 23, 2009 at 1:20pm

According to the attached picture, in negative gram bacteria such as Escherichia, the lipid layers are more than in compare to the positive gram, Phospholipid, lipoprotein, and lipo poly saccharides(LPS), so I think the nano silver particles can pass through of this layers easier than in compare to positive gram bacteria and reach to protein. Also in negative gram, the Peptidoglycan layer is thinner, about 7-8 nanometer, than positive gram, about 20-80 nanometer, so the nano silver can reach to inner membrane easily. Mitochondria is in the inner membrane and there are some groups of protein in mitochondria that they are responsible to enzymes respiratory. As a result the nano silver can produce the free radical and cause to inactivation of these enzymes and finally to death of bacteria.

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Permalink Reply by Vyom Parashar on September 24, 2009 at 6:16pm

Dear Mr.Jaidev

silver nanoparticles does not follow any particular pathway or signalling mechanism to get enter the cell.
Silver nanoparticles entrance in cell can be compared with killing somebody with bullet. One possible mechanism could be phagocytosis. ROS can be after effect once SNPs get through the cell as a responce of bacterial cell.

Permalink Reply by sudania2000 on September 27, 2009 at 11:00am

Dear All,
In our culture we have local traditions of wearing silver rings or chains when there is some injures or fever, I would like to ask about wearing the silver rings will affect the bact. or it has to be nano...Regards

Permalink Reply by Amod Shepal on September 25, 2009 at 7:13pm

Hello,
In case of Viruss e.g. HIV-I, its a site specific (gp 120 protein recepters) and size dependent binding mechanism that inhibits binding of virus to the host cell.

Amod Shepal
Microbiologist,
Pune, India

Permalink Reply by CH.L.R.Jaidev on October 5, 2009 at 11:27am

Dear Amod,
Thanx for your reply. How can a nanoparticles becomes toxic When if the same is in bulk?

Permalink Reply by Amod Shepal on October 6, 2009 at 4:43pm

Hello,
as nanoparticles are smaller, the greater its surface area to volume ratio and higher its chemical reactivity, and biological activity. as greater the chemical reactivity, results increased in production of ROS, including Free radicals- oxidative stress, inflammation, etc... primary mechanism of TOXICITY..
according to sum reports, there are certain nanoparticles tht may move into the sensitive lungs tissues after inhalation, and cause damage tht may leads to chronic breathing problems..
another e.g. is of Carbon Nanotubes, are frequently linked to the asbestos, tht can leads to Mesothelioma, cancer of lining of lungs becoz of asbestos, results observed during experimenting in abdominal cavity of mice using carbon NTs..

Permalink Reply by Sumit Arora on September 26, 2009 at 8:24am

1.Strong interaction with thiol groups present in cell respiratory enzymes in the microbe cell.
2.Interaction with structural proteins and preferential binding with DNA bases to inhibit replication.
3.Binding of the silver ion to free sulphydryl groups in the bacterium or on its surface leading to inactivation of the enzyme phosphomannose Isomerase (essential for biosynthesis of cell wall).
Therefore, it attacks many sites simultaneously and microbes can not get resistant easily against silver.

Permalink Reply by Idah on September 28, 2009 at 6:09am

dear friends,
Who knows about the technical ionization of nanoparticles silver? How they will be ionized when they are put to water without using the electrodes?

Permalink Reply by CH.L.R.Jaidev on October 8, 2009 at 7:28am

I am thankfull to everyone who had participated in this discussion by sharing some valuable information. I am also thankfull for the sugessions and expression of thier views on this discussion. With enriched information in mind and safisfaction in heart I would like to close this discussion. I am thankful to Nanopaprika for giving such a wonderful platform to interact with international nanotech research people.

with regards,
Ch.L.R.Jaidev.

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